Top latest Five attune flow cytometer Urban news
Top latest Five attune flow cytometer Urban news
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Knowledge-enriched characterization of cell populations. Morphological information and facts from photographs can increase into the richness of flow cytometry knowledge. Such as, the determine demonstrates an in any other case common apoptosis assay utilizing Annexin V and PI, incorporating cell imaging to characterize cells in Every inhabitants to reveal morphologically unique features. These insights couldn't have been gained from multiplex staining by itself.
No matter if you’re a rookie or a professional, take a look at our sources to progress your exploration and experience in flow cytometry.
(B) Uncooked 264.seven macrophages will often be annotated inaccurately with regard to particle count due to their complexity and noncircular borders. The volume of singlets readily available for Examination raises after the user trains and employs a far more accurate product.
Find new insights into your experimental information by visualizing populations employing put together fluorescence and morphology details. Our software program interface wizards permit downsampling, concatenation (merged files) and dimensionality instruments that happen to be designed to the application for an intuitive, streamlined info Evaluation working experience.
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Listed here, a highly trained user has gated singlets confidently. Immediately after analyzing the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate consists of greater than four% aggregates.
Pair your Attune flow cytometer with the autosampler for greater workflow effectiveness, and insert robotic integration for maximum throughput.
Steady graphic high quality even at high throughput Acoustic focusing along with a higher-pace camera Incorporate to picture these T read more cells continuously at low or significant flow prices. Easily adjust aim and camera settings to meet experimental demands.
Early indications of this contamination had in the beginning been dismissed as particles. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also means that you can use morphological features to find out intriguing subpopulations that may not be clear from flow cytometry details alone.
Perhaps most significantly, these events consist of cells of clearly various phenotypes which may lead to incorrect conclusions concerning double positive situations (specifically in uncommon populations).
Seem waves help precise cell alignment, causing Extraordinary data excellent and ~10X more rapidly sample flow costs.
Application Take note: Automatic picture analysis lowers person-to-user variability in flow cytometry gating methods
Leverage the strength of Highly developed know-how and innovation to speed up your discoveries. Rely on inside the Attune Flow Cytometer household to provide the trustworthiness, velocity, and precision that established your get the job done apart.
Versatile payment plan from Agilent Fiscal Alternatives assist you to receive the critical instrumentation you may need for quick project, or to develop your lab.
Gating on these extended parameters lets you speedily and accurately recognize populations of desire and confirm gating system with little if any handbook evaluate. The Attune software program offers access to point out-of-the-artwork equipment Discovering Assessment instruments inside of an expertly made person interface, which makes it exceptionally simple to operate.
The Attune Xenith cytometer can be a superior-parameter spectral analyzer, giving both equally spectral unmixing and conventional flow abilities for thorough and functional mobile Assessment.
The Attune CytPix Flow Cytometer will allow users to visualize samples all through acquisition, verify the precision of gating techniques, and support outcomes with responsible documentation for publication functions.
What gave the impression to be a homogeneous population is in fact extra various—an insight that ought to be regarded when interpreting benefits.